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Using ultra-sensitive next generation sequencing to dissect DNA damage-induced mutagenesis.

Scientific reports - 28 Apr 2016

Wang Kaile, Ma Xiaolu, Zhang Xue, Wu Dafei, Sun Chenyi, Sun Yazhou, Lu Xuemei, Wu Chung-I, Guo Caixia, Ruan Jue

Abstract excerpt

Next generation sequencing (NGS) technologies have dramatically improved studies in biology and biomedical science. However, no optimal NGS approach is available to conveniently analyze low frequency mutations caused by DNA damage treatments. Here, by developing an exquisite ultra-sensitive NGS (USNGS) platform "EasyMF" and incorporating it with a widely used supF shuttle vector-based mutagenesis system, we can...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27122023
DOI: 10.1038/srep25310
PMCID: PMC4848531

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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