Article
A dual identification strategy based on padlock ligation and CRISPR/Cas14a for highly specific detection of BRAF V600E mutation in clinical samples.
Analytical methods : advancing methods and applications - 19 May 2022
Shi Weicheng, Gong Yao, Zhang Decai, Yang Tiantian, Yi Ming, Tan Jingyi, Ding Shijia, Cheng Wei
Abstract excerpt
BRAF V600E mutation is a single-nucleotide variation (SNV) that is widely found in various cancers and has been demonstrated to have a strong association with the prognosis and development of some diseases. Thus, we developed a strategy based on rolling circle amplification (RCA) and CRISPR/Cas14a to meet the great need for detecting highly specific BRAF V600E mutation in fine-needle biopsy samples. In this...
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