Article
A novel method to detect mutation in DNA by utilizing exponential amplification reaction triggered by the CRISPR-Cas9 system.
Nanoscale - 21 Apr 2021
Song Jayeon, Kim Soohyun, Kim Hyo Yong, Hur Kyung Hoon, Kim Yoosik, Park Hyun Gyu
Abstract excerpt
We, herein, describe a novel method to detect mutation in DNA by utilizing exponential amplification reaction (EXPAR) triggered by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, called CRISPR-EXPAR. The CRISPR system consisting of two Cas9/sgRNA complexes was designed to cut out a specific mutation region within the target DNA, which would consequently promote EXPAR by continuously...
Topics
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA
- Humans
- Mutation
- RNA, Guide, CRISPR-Cas Systems