Article
Functional analysis of TaPDI genes on storage protein accumulation by CRISPR/Cas9 edited wheat mutants.
International journal of biological macromolecules - 31 Jan 2022
Hu Jinxin, Yu Mei, Chang Yanan, Tang Huali, Wang Wanxin, Du Lipu, Wang Ke, Yan Yueming, Ye Xingguo
Abstract excerpt
Wheat protein disulfide isomerase (PDI) is involved in the formation of glutenin macropolymers (GMP) and the correct folding and accumulation of storage proteins in endosperm. In present study, seven types of homozygous TaPDI gene edited mutants were obtained by CRISPR/Cas9 technology, which were confirmed by PCR-RE and sequencing. Compared with other mutants and wild type (WT), the grain length and width in...
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