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Modification of starch traits in commercial wheat through TaWaxy gene editing.

Carbohydrate polymers - 15 Nov 2025

Qiu Yuliang, Li Xi, Fan Ming, Tang Huali, Zhang Shuangxi, Huang Weihong, Han Zhiyang, Wang Surong, Peng Hao, Xiao Yonggui, Ye Xingguo, Wang Ke

Abstract excerpt

Amylose content (AC) is a key determinant of wheat quality, and the TaWaxy gene determined amylose synthesis with a dose-dependent effect on AC. In this study, the TaWOX5 gene, which significantly enhances wheat transformation efficiency, was combined with CRISPR/SpCas9 system to generate TaWaxy mutants in a commercial winter wheat Jimai 22. Seven transgene-free mutant types were produced, compared to only three...

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PMID: 40912804
DOI: 10.1016/j.carbpol.2025.124105

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