Mutation of the d-hordein gene by RNA-guided Cas9 targeted editing reducing the grain size and changing grain compositions in barley.

doi:10.1016/j.foodchem.2019.125892

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Mutation of the d-hordein gene by RNA-guided Cas9 targeted editing reducing the grain size and changing grain compositions in barley.

Food chemistry - 1 May 2020

Yang Qiang, Zhong Xiaojuan, Li Qing, Lan Jinyu, Tang Huaping, Qi Pengfei, Ma Jian, Wang Jirui, Chen Guoyue, Pu Zhien, Li Wei, Lan Xiujin, Deng Mei, Harwood Wendy, Li Zhongyi, Wei Yuming, Zheng Youliang, Jiang Qiantao

Abstract excerpt

In this study, we successfully knock-out the d-hordein component of barley storage protein using RNA-guided Cas9. Mutation frequencies of 25% and 14% at two different target sites were obtained. Homozygous mutant plants that were T-DNA free were identified in the T1 generation. Barley grains without d-hordein proteins from T2 seeds showed a significantly reduced grain size compared to the parent plant and control...

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Mutation of the d-hordein gene by RNA-guided Cas9 targeted editing reducing the grain size and changing grain compositions in barley.

Abstract excerpt

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Mutation of the d-hordein gene by RNA-guided Cas9 targeted editing reducing the grain size and changing grain compositions in barley.

Abstract excerpt

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