Article
Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method.
Biotechnology letters - 1 Nov 2020
Wei Yan-Yan, Zhan Qun-Mei, Zhu Xiang-Xing, Yan Ai-Fen, Feng Juan, Liu Lian, Li Jian-Hao, Tang Dong-Sheng
Abstract excerpt
OBJECTIVES: Guangdong Small-ear Spotted (GDSS) pigs are a pig breed native to China that possesses unfortunate disadvantages, such as slow growth rate, low lean-meat percentage, and reduced feed utilization. In contrast to traditional genetic breeding methods with long cycle time and high cost, CRISPR/Cas9-mediated gene editing for the modification of the pig genome can quickly improve production traits, and...
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