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Article

Utilization of nicking properties of CRISPR-Cas12a effector for genome editing.

Scientific reports - 9 Feb 2024

Kim Chan Hyoung, Lee Wi-Jae, Oh Yeounsun, Lee Youngjeon, Lee Hyomin K, Seong Jung Bae, Lim Kyung-Seob, Park Sang Je, Huh Jae-Won, Kim Young-Hyun, Kim Kyoung Mi, Hur Junho K, Lee Seung Hwan

Abstract excerpt

The CRISPR-Cas nickase system for genome editing has attracted considerable attention owing to its safety, efficiency, and versatility. Although alternative effectors to Cas9 have the potential to expand the scope of genome editing, their application has not been optimized. Herein, we used an enhanced CRISPR-Cas12a nickase system to induce mutations by targeting genes in a human-derived cell line. The optimized...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 38336977
DOI: 10.1038/s41598-024-53648-2
PMCID: PMC10858195

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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