Article
Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption.
Nucleic acids research - 6 Jul 2018
Gopalappa Ramu, Suresh Bharathi, Ramakrishna Suresh, Kim Hyongbum Henry
Abstract excerpt
The use of paired Cas9 nickases instead of Cas9 nuclease drastically reduces off-target effects. Because both nickases must function for a nickase pair to make a double-strand break, the efficiency of paired nickases can intuitively be expected to be lower than that of either corresponding nuclease alone. Here, we carefully compared the gene-disrupting efficiency of Cas9 paired nickases with that of nucleases....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.