Article

NmeCas9 is an intrinsically high-fidelity genome editing platform

2017-08-04

Abstract excerpt

<h4>ABSTRACT</h4> <h4>Background</h4> The development of CRISPR genome editing has transformed biomedical research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus pyogenes SF370 (SpyCas9). With many RNA guides, wild-type SpyCas9 can induce significant levels of unintended mutations at near-cognate sites, necessitating substantial efforts toward the development of strategies to...

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Literature Corpus work: bdfdd648-22d2-5a8a-9ef9-aeffc8fa5bbc
DOI: 10.1101/172650

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