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Article

Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation.

BMC biotechnology - 27 Jul 2021

Edmondson Carina, Zhou Qi, Liu Xuan

Abstract excerpt

BACKGROUND: The use of CRISPR/Cas9 technologies in generating single-base pair knock-in mutations has recently exploded in the number of methods available. However, with the growing expansion of new technologies, it can be difficult to determine the best method for genome editing. RESULTS: In this study, we evaluated a number of CRISPR/Cas9 approaches for deriving cell lines with knock-in base pair edits to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34315458
DOI: 10.1186/s12896-021-00707-5
PMCID: PMC8317408

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