Article

CRISPR Knock-in Designer: automatic oligonucleotide design software to introduce point mutations using CRISPR/Cas9

2021-04-12

Abstract excerpt

Knock-in of precise point mutations into protein-coding genes has been one of the earliest and most important applications of Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9. The ability to perform such precise gene editing is crucial to interrogate the function of specific protein residues and to create models of human diseases caused by protein amino acid changes. The homologous protein residue...

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Literature Corpus work: e45801a2-f784-5490-80fd-518c3a932c9a
DOI: 10.1101/2021.04.11.439384

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