Article

Highly Efficient Knockin in Human iPS Cells and Rat Embryos by CRISPR/Cas9 Molecular Optimization

2021-09-04

Abstract excerpt

The CRISPR/Cas9 system is now the gold standard for the generation of genetically modified cell and animal models but knockin is a bottleneck. One reason could be that there is no consensus regarding the concentrations of its components to be used. Here, we defined optimal Cas9 protein, guide RNA and short donor DNA concentrations on a GFP to BFP conversion model of human induced pluripotent stem cells and point m...

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Literature Corpus work: 1911464c-06a1-5701-a0b7-1e14d1b48235
DOI: 10.1101/2021.09.03.458921

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