Article
High sensitivity sanger sequencing detection of BRAF mutations in metastatic melanoma FFPE tissue specimens.
Scientific reports - 27 Apr 2021
Cheng Lauren Y, Haydu Lauren E, Song Ping, Nie Jianyi, Tetzlaff Michael T, Kwong Lawrence N, Gershenwald Jeffrey E, Davies Michael A, Zhang David Yu
Abstract excerpt
Mutations in the BRAF gene at or near the p. V600 locus are informative for therapy selection, but current methods for analyzing FFPE tissue DNA generally have a limit of detection of 5% variant allele frequency (VAF), or are limited to the single variant (V600E). These can result in false negatives for samples with low VAFs due to low tumor content or subclonal heterogeneity, or harbor non-V600 mutations. Here,...
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