Article
Calibration-free NGS quantitation of mutations below 0.01% VAF.
Nature communications - 21 Oct 2021
Dai Peng, Wu Lucia Ruojia, Chen Sherry Xi, Wang Michael Xiangjiang, Cheng Lauren Yuxuan, Zhang Jinny Xuemeng, Hao Pengying, Yao Weijie, Zarka Jabra, Issa Ghayas C, Kwong Lawrence, Zhang David Yu
Abstract excerpt
Quantitation of rare somatic mutations is essential for basic research and translational clinical applications including minimal residual disease (MRD) detection. Though unique molecular identifier (UMI) has suppressed errors for rare mutation detection, the sequencing depth requirement is high. Here, we present Quantitative Blocker Displacement Amplification (QBDA) which integrates sequence-selective variant...
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