Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR.

doi:10.1016/j.clinbiochem.2014.12.007

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Article

Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR.

Clinical biochemistry - 1 Oct 2015

Reid Anna L, Freeman James B, Millward Michael, Ziman Melanie, Gray Elin S

Abstract excerpt

OBJECTIVES: Defining the BRAF mutation status in metastatic melanoma patients is critical to selecting patients for therapeutic treatment with targeted therapies. Circulating tumour cells (CTCs) can provide an alternative source of contemporaneous tumour genetic material. However methodologies to...

Topics

Alleles
Amino Acid Substitution
Binding, Competitive
Biomarkers, Tumor
DNA, Neoplasm
Genome-Wide Association Study
Hospitals, Teaching
Humans
Limit of Detection
Melanoma
Microchemistry
Mutant Proteins
Mutation Rate
Neoplastic Cells, Circulating
Nucleic Acid Hybridization
Proto-Oncogene Proteins B-raf
RNA, Neoplasm
Real-Time Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 25523300
DOI: 10.1016/j.clinbiochem.2014.12.007

Genes

BRAF

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-25523300-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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