Article

Detecting and Quantitating Low Fraction DNA Variants with Low-Depth Sequencing

2020-04-28

Abstract excerpt

DNA sequence variants with low allele frequencies below 1% are difficult to detect and quantitate by sequencing, due to the intrinsic error of sequencing-by-synthesis (NGS). Unique molecular identifier barcodes can in principle help NGS detect mutations down to 0.1% variant allele frequency (VAF), but require extremely high sequencing depths of over 25,000x, rendering high sensitivity mutation detection out of rea...

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Literature Corpus work: 8c5fa04b-d54c-5664-840a-403e75d6ad6d
DOI: 10.1101/2020.04.26.061747

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