Article
Quantitative cell-free circulating BRAFV600E mutation analysis by use of droplet digital PCR in the follow-up of patients with melanoma being treated with BRAF inhibitors.
Clinical chemistry - 1 Jan 2015
Sanmamed Miguel F, Fernández-Landázuri Sara, Rodríguez Carmen, Zárate Ruth, Lozano María D, Zubiri Leyre, Perez-Gracia José Luis, Martín-Algarra Salvador, González Alvaro
Abstract excerpt
BACKGROUND: Around 50% of cutaneous melanomas harbor the BRAF(V600E) mutation and can be treated with BRAF inhibitors. DNA carrying this mutation can be released into circulation as cell-free BRAF(V600E) (cfBRAF(V600E)). Droplet digital PCR (ddPCR) is an analytically sensitive technique for quantifying small concentrations of DNA. We studied the plasma concentrations of cfBRAF(V600E) by ddPCR in patients with...
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