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Article

Droplet Digital PCR for Mutation Detection in Formalin-Fixed, Paraffin-Embedded Melanoma Tissues: A Comparison with Sanger Sequencing and Pyrosequencing.

The Journal of molecular diagnostics : JMD - 1 Mar 2018

McEvoy Ashleigh C, Wood Benjamin A, Ardakani Nima M, Pereira Michelle R, Pearce Robert, Cowell Lester, Robinson Cleo, Grieu-Iacopetta Fabienne, Spicer Alexander J, Amanuel Benhur, Ziman Melanie, Gray Elin S

Abstract excerpt

The identification of somatic mutations is crucial for guiding therapeutic decisions about personalized melanoma treatment. However, genetic analysis of tumors is usually performed on limited and often low-quality DNA from tumors with low tumor cellularity and high tumor heterogeneity. Different mutation-detection platforms exist, with varying analytical sensitivities. Here we evaluated the detection of common...

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Source

Identifiers

PMID: 29305225
DOI: 10.1016/j.jmoldx.2017.11.009

Genes

BRAF
NRAS
TERT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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