Article
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Nature biotechnology - 1 Jan 2002
Nagai Takeharu, Ibata Keiji, Park Eun Sun, Kubota Mie, Mikoshiba Katsuhiko, Miyawaki Atsushi
Abstract excerpt
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has provided a myriad of applications for biological systems. Over the last several years, mutagenesis studies have improved folding properties of GFP (refs 1,2). However, slow maturation is still a big obstacle to the use of GFP variants for visualization. These problems are exacerbated when GFP variants are expressed at 37 degrees C and/or...
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