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Article

Engineered dual selection for directed evolution of SpCas9 PAM specificity.

Nature communications - 13 Jan 2021

Goldberg Gregory W, Spencer Jeffrey M, Giganti David O, Camellato Brendan R, Agmon Neta, Ichikawa David M, Boeke Jef D, Noyes Marcus B

Abstract excerpt

The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interactions between its guide RNA and target DNA. Previous reports have established that the PAM specificity of SpCas9 can be altered via positive selection procedures for directed evolution or other protein...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 33441553
DOI: 10.1038/s41467-020-20650-x
PMCID: PMC7807044

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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