Article
Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing.
Nature protocols - 1 Feb 2021
Ellis Peter, Moore Luiza, Sanders Mathijs A, Butler Timothy M, Brunner Simon F, Lee-Six Henry, Osborne Robert, Farr Ben, Coorens Tim H H, Lawson Andrew R J, Cagan Alex, Stratton Mike R, Martincorena Inigo, Campbell Peter J
Abstract excerpt
Somatic mutations accumulate in healthy tissues as we age, giving rise to cancer and potentially contributing to ageing. To study somatic mutations in non-neoplastic tissues, we developed a series of protocols to sequence the genomes of small populations of cells isolated from histological sections. Here, we describe a complete workflow that combines laser-capture microdissection (LCM) with low-input genome...
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