Article

A Universal Duplex Sequencing Approach for Accurate Detection of Somatic Mutations

2025-09-16

Abstract excerpt

Ultra-accurate detection of rare somatic mutations is critical for understanding mutational processes in human disease, aging, and environmental exposures, yet current methods are limited by error rates, restricted genome coverage, and high DNA input. We present UDSeq, a duplex sequencing protocol combining random fragmentation, efficient UMI ligation, and quantitative input control to achieve near-complete genome...

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Literature Corpus work: 09b8f7b8-7a5b-5038-b50f-13646e75c9e2
DOI: 10.1101/2025.09.14.676103

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