Article
Genome-wide quantification of rare somatic mutations in normal human tissues using massively parallel sequencing.
Proceedings of the National Academy of Sciences of the United States of America - 30 Aug 2016
Hoang Margaret L, Kinde Isaac, Tomasetti Cristian, McMahon K Wyatt, Rosenquist Thomas A, Grollman Arthur P, Kinzler Kenneth W, Vogelstein Bert, Papadopoulos Nickolas
Abstract excerpt
We present the bottleneck sequencing system (BotSeqS), a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately before library amplification. We use BotSeqS to show age- and tissue-dependent accumulations of rare mutations and demonstrate that somatic...
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