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Article

Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP.

Scientific reports - 18 Nov 2020

Swain Brendan M, Guo Dawei, Singh Himansha, Rawlins Philip B, McAlister Mark, van Veen Hendrik W

Abstract excerpt

Multidrug transporters can confer drug resistance on cells by extruding structurally unrelated compounds from the cellular interior. In transport assays, Hoechst 33342 (referred to as Hoechst) is a commonly used substrate, the fluorescence of which changes in the transport process. With three basic nitrogen atoms that can be protonated, Hoechst can exist as cationic and neutral species that have different...

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Identifiers

PMID: 33208856
DOI: 10.1038/s41598-020-76943-0
PMCID: PMC7674423

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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