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Article

Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide)

6 Mar 2013

Abstract excerpt

OBJECTIVES: Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342. METHODS:...

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PMID: 23467176
DOI: 10.1093/jac/dkt052

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