Article
The application of pH-sensitive fluorescent dyes in lactic acid bacteria reveals distinct extrusion systems for unmodified and conjugated dyes.
Molecular membrane biology - 1 Jan 2000
Glaasker E, Konings W N, Poolman B
Abstract excerpt
Intracellular pH in bacteria can be measured efficiently between internal pH values of 6.5 and 8.5 with the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5[and-6]-carboxyfluorescein (BCECF). A new fluorescent pH probe with a lower pKa(app) than BCECF was synthesized from fluorescein isothio...
Topics
- Fluorescein-5-isothiocyanate
- Fluoresceins
- Fluorescent Dyes
- Glutamates
- Hydrogen-Ion Concentration
- Indomethacin
- Lactic Acid
- Lactobacillus
- Lactococcus lactis
- Mutation