Article
Energetics of wild-type and mutant multidrug resistance secondary transporter LmrP of Lactococcus lactis.
Biochimica et biophysica acta - 4 Oct 2004
Mazurkiewicz Piotr, Driessen Arnold J M, Konings Wil N
Abstract excerpt
LmrP, a proton/multidrug antiporter of Lactococcus lactis, transports a variety of cationic substrates. Previously, two membrane-embedded acidic residues, Asp142 and Glu327, have been reported to be important for multidrug transport activity of LmrP. Here we show that neither Glu327 nor Asp142 is essential for ethidium binding but that Glu327 is a critical residue for the high affinity binding of Hoechst 33342....
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