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Article

Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilic Sulfolobales and the evasion of lethal gene silencing.

RNA biology - 1 Mar 2021

Zink Isabelle Anna, Fouqueau Thomas, Tarrason Risa Gabriel, Werner Finn, Baum Buzz, Bläsi Udo, Schleper Christa

Abstract excerpt

CRISPR type III systems, which are abundantly found in archaea, recognize and degrade RNA in their specific response to invading nucleic acids. Therefore, these systems can be harnessed for gene knockdown technologies even in hyperthermophilic archaea to study essential genes. We show here the broader usability of this posttranscriptional silencing technology by expanding the application to further essential...

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Source

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Identifiers

PMID: 32957821
DOI: 10.1080/15476286.2020.1813411
PMCID: PMC7951960

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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