Production of a biologically active variant form of recombinant human secretin.

doi:10.1016/0196-9781(88)90264-1

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Article

Production of a biologically active variant form of recombinant human secretin.

Peptides - 1 Jan 2000

Olson H, Lind P, Pohl G, Henrichson C, Mutt V, Jörnvall H, Josephson S, Uhlén M, Lake M

Abstract excerpt

A biologically active variant form of recombinant human secretin was produced using a gene fusion system designed to facilitate the purification of the protein. The fusion protein was recovered from the culture medium of Escherichia coli by IgG affinity chromatography, and recombinant secretin wa...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Escherichia coli
Genetic Variation
Humans
Molecular Sequence Data
Peptide Fragments
Plasmids
Recombinant Proteins
Secretin
Swine
Trypsin

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