Article
Synthesis and secretion of a fibrinolytically active tissue-type plasminogen activator variant in Escherichia coli.
Gene - 15 Mar 1991
Waldenström M, Holmgren E, Attersand A, Kalderén C, Löwenadler B, Rådén B, Hansson L, Pohl G
Abstract excerpt
A gene encoding a variant (lacking amino acids 6-173) of human tissue-type plasminogen activator (t-PA), consisting only of the second kringle domain (K2) and the serine protease domain (P), was fused to a DNA segment coding for the signal peptide of staphylococcal protein A and a synthetic gene...
Topics
- Amino Acid Sequence
- Chromosome Mapping
- Cloning, Molecular
- DNA, Recombinant
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genetic Variation
- Molecular Sequence Data
- Plasmids
- Recombinant Proteins
- Tissue Plasminogen Activator
- Transformation, Genetic