Article
Biological activities and secondary structures of variant forms of human salivary cystatin SN produced in Escherichia coli.
Gene - 30 Dec 1994
Bobek L A, Ramasubbu N, Wang X, Weaver T R, Levine M J
Abstract excerpt
Using an Escherichia coli expression system, pGEX-2T, that expresses foreign sequences as fusion proteins with a glutathione S-transferase (GST) carrier, we have produced several recombinant human salivary cystatin SN (reCsnSN) variants. These include a N-terminal-truncated form (aa 17-121), a C-...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cathepsin B
- Cattle
- Circular Dichroism
- Cloning, Molecular
- Cystatins
- Escherichia coli
- Genetic Variation
- Glutathione Transferase
- Humans
- Kinetics
- Molecular Sequence Data
- Papain
- Plasmids
- Protease Inhibitors
- Protein Structure, Secondary