Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression, refolding, and purification of recombinant human granzyme B.

Protein expression and purification - 1 Jan 2005

Lorentsen Rikke H, Fynbo Charlotte H, Thøgersen Hans C, Etzerodt Michael, Holtet Thor L

Abstract excerpt

Granzyme B (GrB) is a member of a family of serine proteases involved in cytotoxic T-lymphocyte-mediated killing of potentially harmful cells, where GrB induces apoptosis by cleavage of a limited number of substrates. To investigate the suitability of GrB as an enzyme for specific fusion protein cleavage, two derivatives of human GrB, one dependent on blood coagulation factor Xa (FXa) cleavage for activation and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Granzymes

Share to Kinetics

Share to Mutation

Share to Recombinant Fusion Proteins

Share to Serine Endopeptidases

Share to Substrate Specificity

Source

Identifiers

PMID: 15596356
DOI: 10.1016/j.pep.2004.08.017

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression, refolding, and purification of recombinant human granzyme B.PMID 15596356Read ↗

Design and characterization of a novel human Granzyme B inhibitor.Semantic proximity Read ↗

Identification and characterization of equine granzyme B.Semantic proximity Read ↗

The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity.Semantic proximity Read ↗

Controversies in granzyme biologySemantic proximity Read ↗

Design of human granzyme B variants resistant to serpin B9.Semantic proximity Read ↗

The major human and mouse granzymes are structurally and functionally divergent.Semantic proximity Read ↗

Intracellular serine protease inhibitor SERPINB4 inhibits granzyme M-induced cell death.Semantic proximity Read ↗

A triple-mutated allele of granzyme B incapable of inducing apoptosis.Semantic proximity Read ↗

Purified Zymogens Reveal Mechanisms of Snake Venom Metalloproteinase Auto-ActivationSemantic proximity Read ↗

Structural Insights into the Catalytic Mechanism of Granzyme B upon Substrate and Inhibitor BindingSemantic proximity Read ↗

Characterization of structural determinants of granzyme B reveals potent mediators of extended substrate specificity.Semantic proximity Read ↗

Granzyme B encoded by the commonly occurring human RAH allele retains pro-apoptotic activity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.