Production of recombinant notechis 11'2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli.

Abstract excerpt

We have constructed an expression vector to produce, in Escherichia coli, a fusion protein containing successively two IgG binding domains from staphyloccocal protein A, a nine-amino-acid linker peptide terminating in a methionine residue and the phospholipase A2 notechis 11'2L, an isoform of not...

Topics

• Base Sequence
• Elapid Venoms
• Escherichia coli
• Molecular Sequence Data
• Mutation
• Phospholipases A
• Phospholipases A2
• Protein Folding
• Recombinant Fusion Proteins