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Article

Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli.

Protein expression and purification - 1 Jul 2006

Király Orsolya, Guan Lan, Szepessy Edit, Tóth Miklós, Kukor Zoltán, Sahin-Tóth Miklós

Abstract excerpt

High-level expression of human trypsinogens as inclusion bodies in Escherichia coli requires deletion of the secretory signal sequence and placement of an initiator methionine at the N terminus. Trypsinogen preparations obtained this way contain a mixture of abnormal N termini, as a result of processing by cytoplasmic aminopeptidases. Here, we describe an expression system that produces recombinant human cationic...

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Identifiers

PMID: 16542853
DOI: 10.1016/j.pep.2006.01.023
PMCID: PMC1604731

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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