Article
Genetically engineered proinsulin constitutively processed and secreted as mature, active insulin.
The Journal of biological chemistry - 25 Feb 1994
Groskreutz D J, Sliwkowski M X, Gorman C M
Abstract excerpt
The conversion of human proinsulin to insulin occurs only in specialized cells which contain the appropriate processing enzymes. To allow proinsulin processing to occur in a wide variety of cell types, we engineered human proinsulin to be cleaved in the constitutive secretory pathway. Using site-...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA, Complementary
- Humans
- Insulin
- Insulin Secretion
- Mice
- Molecular Sequence Data
- Mutation
- Phosphorylation
- Proinsulin
- Protein Processing, Post-Translational
- Receptor, Insulin
- Recombinant Proteins