Article
Myostatin site-directed mutation and simultaneous PPARγ site-directed knockin in bovine genome.
Journal of cellular physiology - 1 Apr 2021
Ge Luxing, Kang Jian, Dong Xiangchen, Luan Deji, Su Guanghua, Li Guangpeng, Zhang Yong, Quan Fusheng
Abstract excerpt
Most studies on the acquisition of advantageous traits in transgenic animals only focus on monogenic traits. In practical applications, transgenic animals need to possess multiple advantages. Therefore, multiple genes need to be edited simultaneously. CRISPR/Cas9 technology has been widely used in many research fields. However, few studies on endogenous gene mutation and simultaneous exogenous gene insertion...
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