Article
A MSTNDel73C mutation with FGF5 knockout sheep by CRISPR/Cas9 promotes skeletal muscle myofiber hyperplasia.
eLife - 4 Oct 2024
Chen Ming-Ming, Zhao Yue, Yu Kun, Xu Xue-Ling, Zhang Xiao-Sheng, Zhang Jin-Long, Wu Su-Jun, Liu Zhi-Mei, Yuan Yi-Ming, Guo Xiao-Fei, Qi Shi-Yu, Yi Guang, Wang Shu-Qi, Li Huang-Xiang, Wu Ao-Wu, Liu Guo-Shi, Deng Shou-Long, Han Hong-Bing, Lv Feng-Hua, Lian Di, Lian Zheng-Xing
Abstract excerpt
Mutations in the well-known Myostatin (MSTN) produce a 'double-muscle' phenotype, which makes it commercially invaluable for improving livestock meat production and providing high-quality protein for humans. However, mutations at different loci of the MSTN often produce a variety of different phenotypes. In the current study, we increased the delivery ratio of Cas9 mRNA to sgRNA from the traditional 1:2 to 1:10,...
Topics
- Animals
- Myostatin
- Sheep
- CRISPR-Cas Systems
- Fibroblast Growth Factor 5
- Muscle Fibers, Skeletal
- Mutation
- Gene Knockout Techniques
- Hyperplasia
- Muscle, Skeletal