Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System.

doi:10.1038/srep16623

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Article

Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System.

Scientific reports - 13 Nov 2015

Wang Kankan, Ouyang Hongsheng, Xie Zicong, Yao Chaogang, Guo Nannan, Li Mengjing, Jiao Huping, Pang Daxin

Abstract excerpt

Genetically modified pigs are increasingly used for biomedical and agricultural applications. The efficient CRISPR/Cas9 gene editing system holds great promise for the generation of gene-targeting pigs without selection marker genes. In this study, we aimed to disrupt the porcine myostatin (MSTN) gene, which functions as a negative regulator of muscle growth. The transfection efficiency of porcine fetal...

Topics

Animals
Animals, Genetically Modified
Base Sequence
Blotting, Western
CRISPR-Cas Systems
Cells, Cultured
Cloning, Organism
Fetus
Fibroblasts
Gene Knockout Techniques
Gene Targeting

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Microscopy, Fluorescence

Molecular Sequence Data

Mutation

Myostatin

Reproducibility of Results

Reverse Transcriptase Polymerase Chain Reaction

Swine

Source

Open in PubMed Open full text

Identifiers

PMID: 26564781
DOI: 10.1038/srep16623
PMCID: PMC4643223

Genes

MSTN

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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