Article
A Rapid, SuperSelective Method for Detection of Single Nucleotide Variants in Caenorhabditis elegans.
Genetics - 1 Oct 2020
Touroutine Denis, Tanis Jessica E
Abstract excerpt
With the widespread use of single nucleotide variants generated through mutagenesis screens and genome editing technologies, there is pressing need for an efficient and low-cost strategy to genotype single nucleotide substitutions. We have developed a rapid and inexpensive method for detection of point mutants through optimization of SuperSelective (SS) primers for end-point PCR in Caenorhabditis elegans Each SS...
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