Article
Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping.
Human gene therapy - 1 Jun 2016
Lee Han B, Schwab Tanya L, Koleilat Alaa, Ata Hirotaka, Daby Camden L, Cervera Roberto Lopez, McNulty Melissa S, Bostwick Hannah S, Clark Karl J
Abstract excerpt
Customizable endonucleases such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) enable rapid generation of mutant strains at genomic loci of interest in animal models and cell lines. With the accelerated pace of generating mutant alleles, genotyping has become a rate-limiting step to understanding...
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