Isolation of C. elegans deletion mutants following ENU mutagenesis and thermostable restriction enzyme PCR screening.

Abstract excerpt

The ability to generate null mutants is essential for studying gene function. Gene knockouts in Caenorhabditis elegans can be generated in a high throughput manner using chemical mutagenesis followed by polymerase chain reaction (PCR) assays to detect deletions in a gene of interest. However, current methods for identifying deletions are time and labor intensive and are unable to efficiently detect small...

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