Article
Enhancing Cohort PASA Efficiency from Lessons Assimilated by Mutant Genotyping in C. elegans.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Pandey Amita, Bhat Binu, Aggarwal Madan L, Pandey Girdhar K
Abstract excerpt
Classical restriction fragment length polymorphism (RFLP) and sequencing are labor-intensive and expensive methods to study single base changes, whereas polymerase chain reaction amplification of specific alleles (PASA) or allele-specific polymerase chain reaction (ASPCR) is a PCR-based application that allows direct detection of any point mutation by analyzing the PCR products in an ethidium bromide-stained...
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