Article
Improved plant cytosine base editors with high editing activity, purity, and specificity.
Plant biotechnology journal - 1 Oct 2021
Ren Qiurong, Sretenovic Simon, Liu Guanqing, Zhong Zhaohui, Wang Jiaheng, Huang Lan, Tang Xu, Guo Yachong, Liu Li, Wu Yuechao, Zhou Jie, Zhao Yuxin, Yang Han, He Yao, Liu Shishi, Yin Desuo, Mayorga Rocio, Zheng Xuelian, Zhang Tao, Qi Yiping, Zhang Yong
Abstract excerpt
Cytosine base editors (CBEs) are great additions to the expanding genome editing toolbox. To improve C-to-T base editing in plants, we first compared seven cytidine deaminases in the BE3-like configuration in rice. We found A3A/Y130F-CBE_V01 resulted in the highest C-to-T base editing efficiency in both rice and Arabidopsis. Furthermore, we demonstrated this A3A/Y130F cytidine deaminase could be used to improve...
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