Article
A large-scale genome and transcriptome sequencing analysis reveals the mutation landscapes induced by high-activity adenine base editors in plants.
Genome biology - 9 Feb 2022
Li Shaofang, Liu Lang, Sun Wenxian, Zhou Xueping, Zhou Huanbin
Abstract excerpt
BACKGROUND: The high-activity adenine base editors (ABEs), engineered with the recently-developed tRNA adenosine deaminases (TadA8e and TadA9), show robust base editing activity but raise concerns about off-target effects. RESULTS: In this study, we perform a comprehensive evaluation of ABE8e- and ABE9-induced DNA and RNA mutations in Oryza sativa. Whole-genome sequencing analysis of plants transformed with four...
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