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Article

Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles.

Nature methods - 1 Jul 2020

Michlits Georg, Jude Julian, Hinterndorfer Matthias, de Almeida Melanie, Vainorius Gintautas, Hubmann Maria, Neumann Tobias, Schleiffer Alexander, Burkard Thomas Rainer, Fellner Michaela, Gijsbertsen Max, Traunbauer Anna, Zuber Johannes, Elling Ulrich

Abstract excerpt

CRISPR-Cas9 screens have emerged as a transformative approach to systematically probe gene functions. The quality and success of these screens depends on the frequencies of loss-of-function alleles, particularly in negative-selection screens widely applied for probing essential genes. Using optimized screening workflows, we performed essentialome screens in cancer cell lines and embryonic stem cells and achieved...

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PMID: 32514112
DOI: 10.1038/s41592-020-0850-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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