Article
Computational correction of copy number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells.
Nature genetics - 1 Dec 2017
Meyers Robin M, Bryan Jordan G, McFarland James M, Weir Barbara A, Sizemore Ann E, Xu Han, Dharia Neekesh V, Montgomery Phillip G, Cowley Glenn S, Pantel Sasha, Goodale Amy, Lee Yenarae, Ali Levi D, Jiang Guozhi, Lubonja Rakela, Harrington William F, Strickland Matthew, Wu Ting, Hawes Derek C, Zhivich Victor A, Wyatt Meghan R, Kalani Zohra, Chang Jaime J, Okamoto Michael, Stegmaier Kimberly, Golub Todd R, Boehm Jesse S, Vazquez Francisca, Root David E, Hahn William C, Tsherniak Aviad
Abstract excerpt
The CRISPR-Cas9 system has revolutionized gene editing both at single genes and in multiplexed loss-of-function screens, thus enabling precise genome-scale identification of genes essential for proliferation and survival of cancer cells. However, previous studies have reported that a gene-independent antiproliferative effect of Cas9-mediated DNA cleavage confounds such measurement of genetic dependency, thereby...
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