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Article

The mutational impact of culturing human pluripotent and adult stem cells.

Nature communications - 19 May 2020

Kuijk Ewart, Jager Myrthe, van der Roest Bastiaan, Locati Mauro D, Van Hoeck Arne, Korzelius Jerome, Janssen Roel, Besselink Nicolle, Boymans Sander, van Boxtel Ruben, Cuppen Edwin

Abstract excerpt

Genetic changes acquired during in vitro culture pose a risk for the successful application of stem cells in regenerative medicine. To assess the genetic risks induced by culturing, we determined all mutations in individual human stem cells by whole genome sequencing. Individual pluripotent, intestinal, and liver stem cells accumulate 3.5 ± 0.5, 7.2 ± 1.1 and 8.3 ± 3.6 base substitutions per population doubling,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32427826
DOI: 10.1038/s41467-020-16323-4
PMCID: PMC7237696

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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