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Article

Measuring mutation accumulation in single human adult stem cells by whole-genome sequencing of organoid cultures.

Nature protocols - 1 Jan 2018

Jager Myrthe, Blokzijl Francis, Sasselli Valentina, Boymans Sander, Janssen Roel, Besselink Nicolle, Clevers Hans, van Boxtel Ruben, Cuppen Edwin

Abstract excerpt

Characterization of mutational processes in adult stem cells (ASCs) will improve our understanding of aging-related diseases, such as cancer and organ failure, and may ultimately help prevent the development of these diseases. Here, we present a method for cataloging mutations in individual human ASCs without the necessity of using error-prone whole-genome amplification. Single ASCs are expanded in vitro into...

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Identifiers

PMID: 29215633
DOI: 10.1038/nprot.2017.111

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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