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Pitfalls in Single Clone CRISPR-Cas9 Mutagenesis to Fine-map Regulatory Intervals.

Genes - 4 May 2020

Tian Ruoyu, Pan Yidan, Etheridge Thomas H A, Deshmukh Harshavardhan, Gulick Dalia, Gibson Greg, Bao Gang, Lee Ciaran M

Abstract excerpt

The majority of genetic variants affecting complex traits map to regulatory regions of genes, and typically lie in credible intervals of 100 or more SNPs. Fine mapping of the causal variant(s) at a locus depends on assays that are able to discriminate the effects of polymorphisms or mutations on gene expression. Here, we evaluated a moderate-throughput CRISPR-Cas9 mutagenesis approach, based on replicated...

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Identifiers

PMID: 32375333
DOI: 10.3390/genes11050504
PMCID: PMC7288657

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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